6B0F
ESTROGEN RECEPTOR ALPHA LIGAND BINDING DOMAIN IN COMPLEX WITH LSZ102
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.820, 52.100, 97.020 |
| Unit cell angles | 90.00, 114.33, 90.00 |
Refinement procedure
| Resolution | 45.840 - 2.860 |
| R-factor | 0.231 |
| Rwork | 0.227 |
| R-free | 0.29900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1MTYS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BUSTER (2.11.7) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.210 | 2.870 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Rmerge | 0.117 | 0.583 |
| Number of reflections | 11212 | 107 |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 98.8 | 99.1 |
| Redundancy | 3.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1 M TRIS-HCL (PH 8.5), 22% PEG 3350, 0.20 M MAGNESIUM CHLORIDE |
