6AXJ
Crystal structure of the Yaf9 YEATS domain bound to H3K27ac
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-16 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 140.318, 140.318, 129.054 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.079 - 2.379 |
| R-factor | 0.1814 |
| Rwork | 0.179 |
| R-free | 0.22690 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.964 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.100 |
| High resolution limit [Å] | 2.379 |
| Number of reflections | 37536 |
| <I/σ(I)> | 11.5 |
| Completeness [%] | 98.8 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.14 | 2.0 M Ammonium Sulfate, 0.1 M Bis-Tris |
