6AXF

Structure of RasGRP2 in complex with Rap1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	I 21 21 21
Unit cell lengths	138.998, 209.258, 336.765
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.460 - 3.100
R-factor	0.2052
Rwork	0.203
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.550
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.460
High resolution limit [Å]	3.100
Rmerge	0.148
Number of reflections	171499
<I/σ(I)>	6.7
Completeness [%]	100.0
Redundancy	4.5
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	17% PEG3350 and 10 mM sodium citrate (pH 5.6)