6AU6

Crystal structure of GDP-bound human GNAS R201C mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999907
Spacegroup name	P 21 21 21
Unit cell lengths	37.370, 85.580, 125.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.042 - 1.700
R-factor	0.1701
Rwork	0.168
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1azt
RMSD bond length	0.005
RMSD bond angle	0.859
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.820	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.082	0.342
Rpim	0.035	0.149
Number of reflections	44469	6189
<I/σ(I)>	12	3.8
Completeness [%]	98.4	95.7
Redundancy	6.1	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris 8.5, 25% PEG8000, 10 mM TCEP hydrochloride