6AT0
Chromodomain HP1 with a p-nitro-L-phenylalanine mutation at position 24 bound to histone H3 peptide containing trimethyl lysine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 34.417, 76.857, 76.479 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 11.224 - 1.285 |
R-factor | 0.2418 |
Rwork | 0.241 |
R-free | 0.25970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kne |
RMSD bond length | 0.004 |
RMSD bond angle | 0.751 |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 11.224 |
High resolution limit [Å] | 1.285 |
Number of reflections | 25429 |
<I/σ(I)> | 12.8 |
Completeness [%] | 96.9 |
Redundancy | 5.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 277 | 0.1M MES, 3.0M (NH4)2SO4 |