6AO5

Crystal structure of human MST2 in complex with SAV1 SARAH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	H 3 2
Unit cell lengths	223.678, 223.678, 79.645
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.271 - 2.955
R-factor	0.2357
Rwork	0.235
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lgd
RMSD bond length	0.009
RMSD bond angle	0.600
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.950	8.000	2.950
Rmerge	0.124	0.051	1.715
Rmeas	0.128	0.053	1.788
Rpim	0.029	0.012	0.483
Total number of observations	303392
Number of reflections	15976	839	763
<I/σ(I)>	7
Completeness [%]	99.9	99.3	98.5
Redundancy	19	18.4	12
CC(1/2)		0.999	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.05 M NaCl, 0.1 M Hepes, 0.19 mM CYMAL-7, 1 mM TCEP, 40% PEG 400