6AJ4
Crystal structure of the DHR-2 domain of DOCK7 in complex with Cdc42
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 196.460, 105.560, 118.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.802 - 3.256 |
| R-factor | 0.1876 |
| Rwork | 0.184 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vhl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.289 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.450 |
| High resolution limit [Å] | 3.256 | 3.260 |
| Rmeas | 0.120 | 0.960 |
| Number of reflections | 73997 | 11796 |
| <I/σ(I)> | 11.9 | 1.7 |
| Completeness [%] | 99.4 | 97.8 |
| Redundancy | 3.9 | |
| CC(1/2) | 0.996 | 0.639 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350 and tri-potassium citrate monohydrate |
