6AEX

Crystal structure of unoccupied murine uPAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.04
Spacegroup name	P 41 21 2
Unit cell lengths	83.820, 83.820, 81.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.261 - 2.393
R-factor	0.1976
Rwork	0.195
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3laq
RMSD bond length	0.008
RMSD bond angle	1.011
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.261	2.479
High resolution limit [Å]	2.393	2.393
Rmerge	0.094	0.786
Number of reflections	11915	585
<I/σ(I)>	45.5
Completeness [%]	99.7
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.4	293	50mM Tris.Cl, 3M NaCl