6ACI
Crystal structure of EPEC effector NleB in complex with FADD death domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-11-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 84.590, 84.590, 145.881 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.589 - 1.870 |
| R-factor | 0.1666 |
| Rwork | 0.164 |
| R-free | 0.20940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e66 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.603 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.630 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.091 | 0.473 |
| Number of reflections | 50430 | 7314 |
| <I/σ(I)> | 16.3 | 5.5 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 10.4 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 4.5 M Sodium chloride, 100 mM HEPES pH 7.5 |
