6A6J

Crystal structure of Zebra fish Y-box protein1 (YB-1) Cold-shock domain in complex with 6mer m5C RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97914
Spacegroup name	C 1 2 1
Unit cell lengths	62.201, 60.866, 56.513
Unit cell angles	90.00, 118.97, 90.00

Refinement procedure

Resolution	27.210 - 2.255
R-factor	0.2295
Rwork	0.225
R-free	0.27240
RMSD bond length	0.004
RMSD bond angle	1.133
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.330
High resolution limit [Å]	2.250	2.250
Number of reflections	7519
<I/σ(I)>	7.38
Completeness [%]	94.6
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.05M Zinc acetate, 22% PEG 3350