6A47
Structure of TREX2 in complex with a Y structured dsDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-04 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.666, 48.404, 90.593 |
Unit cell angles | 90.00, 97.36, 90.00 |
Refinement procedure
Resolution | 28.454 - 1.900 |
R-factor | 0.1653 |
Rwork | 0.163 |
R-free | 0.19110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y97 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.956 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.043 | 0.453 |
Rpim | 0.024 | 0.274 |
Number of reflections | 50026 | 4963 |
<I/σ(I)> | 33.34 | 2.77 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 3.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1M Sodium citrate tribasic dihydrate pH 5.0, 30%(v/v) Polyethylene glycol monomethyl ether 550, D-(+)-Glucose monohydrate |