5ZXF
The 1.25A Crystal structure of His6-tagged Mdm2 in complex with nutlin-3a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.575, 43.222, 53.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.420 - 1.250 |
R-factor | 0.2191 |
Rwork | 0.218 |
R-free | 0.24310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.806 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.6.2) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.420 | 1.320 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.073 | 0.648 |
Rmeas | 0.077 | 0.702 |
Rpim | 0.023 | 0.261 |
Number of reflections | 27731 | 3735 |
<I/σ(I)> | 20.2 | |
Completeness [%] | 98.8 | 93 |
Redundancy | 11.7 | 6.8 |
CC(1/2) | 0.996 | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M NaOAc, 1.2M AmSO4, Seeding. |