5ZNR
Crystal structure of PtSHL in complex with an H3K27me3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.717, 110.315, 58.643 |
Unit cell angles | 90.00, 96.64, 90.00 |
Refinement procedure
Resolution | 40.051 - 3.202 |
R-factor | 0.209 |
Rwork | 0.206 |
R-free | 0.23210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5znp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.156 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.190 | 0.786 |
Number of reflections | 9089 | 904 |
<I/σ(I)> | 5.4 | 1.2 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 277 | 0.2 M lithium sulfate, 2.0 M ammonium sulfate, 0.1 M CAPS, pH 10.5 |