5ZNN
Crystal structure of ligand-free form of the Vps10 ectodomain of Sortilin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-05 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.264, 138.496, 146.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.607 - 2.448 |
R-factor | 0.1814 |
Rwork | 0.180 |
R-free | 0.21680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4po7 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.448 | 2.450 |
Number of reflections | 60757 | 2997 |
<I/σ(I)> | 22.6 | 2.5 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 4.6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 23% (w/v) polyethylene glycol monomethyl ether 550 (Sigma), 0.4 M sodium acetate pH 4.6 |