5ZMJ
Crystal structure of the Fab region of a neutralizing fully human antibody against GM-CSF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.120, 66.690, 129.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.370 - 1.810 |
R-factor | 0.16272 |
Rwork | 0.161 |
R-free | 0.19133 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eow |
RMSD bond length | 0.005 |
RMSD bond angle | 0.796 |
Data reduction software | iMOSFLM |
Data scaling software | pointless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.370 | 1.850 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.068 | 0.171 |
Number of reflections | 43756 | 3133 |
<I/σ(I)> | 27.2 | |
Completeness [%] | 98.1 | |
Redundancy | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2M Potassium sodium tartrate tetrahydrate, 0.1M Sodium citrate tribasic dihydrate pH 5.6, 2.0M Ammonium sulfate |