5ZMJ
Crystal structure of the Fab region of a neutralizing fully human antibody against GM-CSF
Summary for 5ZMJ
| Entry DOI | 10.2210/pdb5zmj/pdb |
| Descriptor | Heavy chain of a Fab fraction of human IgG, Light chain of a Fab fraction of human IgG, SULFATE ION, ... (5 entities in total) |
| Functional Keywords | fab, fully human antibody, gm-csf, immune system |
| Biological source | Homo sapiens More |
| Total number of polymer chains | 2 |
| Total formula weight | 52372.14 |
| Authors | Angkawidjaja, C.,Torashima, T. (deposition date: 2018-04-04, release date: 2019-04-17, Last modification date: 2024-11-13) |
| Primary citation | Angkawidjaja, C.,Torashima, T. Crystal structure of the Fab region of a neutralizing antibody against granulocyte-macrophage colony-stimulating factor. Acta Crystallogr.,Sect.F, 75:634-639, 2019 Cited by PubMed Abstract: An increased level of granulocyte-macrophage colony-stimulating factor has a potential role in the development of autoimmune diseases, and the neutralization of its activity by monoclonal antibodies is a promising therapy for some diseases. Here, the crystal structure of the Fab region of EV1007, a fully human antibody expressed in Chinese hamster ovary cells that was developed from human peripheral blood mononuclear cells, is described. The structure closely resembles that of MB007, which is the Fab region of the same antibody expressed in Escherichia coli [Blech et al. (2012), Biochem. J. 447, 205-215], except at the hinge regions between the immunoglobulin domains and the H3 loop region. This paper presents evidence for the flexibility of the hinge and H3 loop regions of the antibody based on the comparison of two independently solved crystal structures. PubMed: 31584011DOI: 10.1107/S2053230X1901238X PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.81 Å) |
Structure validation
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