5ZM8

Crystal structure of ORP2-ORD in complex with PI(4,5)P2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97775
Spacegroup name	I 4 2 2
Unit cell lengths	156.160, 156.160, 199.221
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.322 - 2.700
R-factor	0.2178
Rwork	0.216
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zm5
RMSD bond length	0.008
RMSD bond angle	1.078
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.122	0.553
Rmeas	0.129	0.588
Rpim	0.042	0.198
Number of reflections	34082	1676
<I/σ(I)>	17.8	4.38
Completeness [%]	100.0	99.8
Redundancy	9.9	8.6
CC(1/2)		0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	100mM MES pH 6.5, 50mM MgCl2, 8% isopropanol, 1.5% PEG3350