5ZJE
LDHA-mla
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.588, 219.333, 112.788 |
| Unit cell angles | 90.00, 92.28, 90.00 |
Refinement procedure
| Resolution | 30.190 - 2.929 |
| R-factor | 0.1928 |
| Rwork | 0.188 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4okn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.241 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.200 | 2.962 |
| High resolution limit [Å] | 2.929 | 2.929 |
| Rmerge | 0.168 | 0.385 |
| Number of reflections | 86824 | 1694609 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 97.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.2M soduim malonnate, 20% Polyethylene glycol(PEG) 3350 |
