5ZG3
Crystal structure of the GluA2o LBD in complex with glutamate and TAK-137
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976486 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 114.065, 162.332, 47.385 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.650 |
R-factor | 0.18571 |
Rwork | 0.184 |
R-free | 0.21486 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ftj |
RMSD bond length | 0.010 |
RMSD bond angle | 1.339 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 205163 | 10200 |
<I/σ(I)> | 19 | 1.5 |
Completeness [%] | 99.6 | 99.1 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.9 | 277 | 15% PEG 3350, 0.1M SODIUM ACETATE, 0.1M ZINC ACETATE |