5ZG2
Crystal structure of the GluA2o LBD in complex with ZK200775 and Compound-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976486 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.384, 56.565, 90.779 |
Unit cell angles | 90.00, 91.74, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.250 |
R-factor | 0.17053 |
Rwork | 0.169 |
R-free | 0.19034 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ftj |
RMSD bond length | 0.011 |
RMSD bond angle | 1.499 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Number of reflections | 128359 | 4044 |
<I/σ(I)> | 19.5 | 1.8 |
Completeness [%] | 93.1 | 59 |
Redundancy | 3.3 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.9 | 277 | 15% PEG 3350, 0.1 M SODIUM ACETATE, 0.1 M ZINC ACETATE |