5ZG0
Crystal structure of the GluA2o LBD in complex with glutamate and Compound-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976486 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 113.682, 162.582, 47.383 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.580 |
R-factor | 0.19484 |
Rwork | 0.193 |
R-free | 0.22058 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ftj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.355 |
Data reduction software | HKL-2000 |
Data scaling software | cctbx.prime |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Number of reflections | 222823 | 10721 |
<I/σ(I)> | 17.9 | 1.4 |
Completeness [%] | 95.2 | 92.9 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.9 | 277 | 15% PEG 3350, 0.1M SODIUM ACETATE, 0.1M ZINC ACETATE |