5ZFW
Crystal structure of human DUX4 homeodomains bound to A11G DNA mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977776 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.748, 64.748, 153.484 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.828 - 2.103 |
R-factor | 0.1941 |
Rwork | 0.192 |
R-free | 0.22720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z6z |
RMSD bond length | 0.004 |
RMSD bond angle | 0.706 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 22217 | |
<I/σ(I)> | 24 | |
Completeness [%] | 98.9 | |
Redundancy | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 0.1 M Sodium malonate pH 4.0, 12% Polyethylene glycol 3350 |