5ZF6
Crystal structure of the dimeric human PNPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2011-02-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.335, 109.334, 84.501 |
Unit cell angles | 90.00, 117.41, 90.00 |
Refinement procedure
Resolution | 42.157 - 2.796 |
R-factor | 0.1967 |
Rwork | 0.193 |
R-free | 0.24890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u1k |
RMSD bond length | 0.003 |
RMSD bond angle | 0.540 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.157 | 2.890 |
High resolution limit [Å] | 2.796 | 2.796 |
Rmerge | 0.081 | 0.570 |
Number of reflections | 32471 | 1594 |
<I/σ(I)> | 15.03 | |
Completeness [%] | 99.9 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.1M citrate buffer (pH 5.0), 10% (v/v) 2-propanol, 26% (v/v) polyethylene glycol 400 |