5ZBQ
The Crystal Structure of human neuropeptide Y Y1 receptor with UR-MK299
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.840, 100.680, 83.160 |
Unit cell angles | 90.00, 98.76, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.166 | 0.937 |
Number of reflections | 16689 | 2119 |
<I/σ(I)> | 4.78 | 1 |
Completeness [%] | 97.3 | 96.9 |
Redundancy | 3.97 | 3.64 |
CC(1/2) | 0.988 | 0.633 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7.4 | 293 | 0.1 M Tris, pH 7.4-8.0, 30-40% (v/v) PEG400, 50-150 mM sodium tartrate and 100 uM UR-MK299 |