5YZC

Crystal structure of the prefusion form of measles virus fusion protein in complex with a fusion inhibitor compound (AS-48)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-23
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	I 21 3
Unit cell lengths	169.497, 169.497, 169.497
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.600 - 2.334
R-factor	0.1856
Rwork	0.184
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yxw
RMSD bond length	0.008
RMSD bond angle	0.937
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11_2567))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.852	2.342
High resolution limit [Å]	2.334	2.334
Number of reflections	34515
<I/σ(I)>	14.3
Completeness [%]	100.0
Redundancy	20.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium formate, glycerol, sodium acetate