5YVX
Crystal structure of SDG8 CW domain in complex with H3K4me1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-03 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.2824 |
| Spacegroup name | P 63 |
| Unit cell lengths | 66.421, 66.421, 29.241 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.761 - 1.591 |
| R-factor | 0.1692 |
| Rwork | 0.168 |
| R-free | 0.18450 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.991 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Number of reflections | 19136 | |
| <I/σ(I)> | 26.6 | |
| Completeness [%] | 98.6 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tris pH 8.5, 30% PEG 3350, 30% iso-propanol |
