5YVI

Crystal structure of Karyopherin beta2 in complex with FUS(456-526)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2012-11-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	129.042, 156.102, 68.248
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.904 - 2.900
R-factor	0.1816
Rwork	0.179
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fdd
RMSD bond length	0.008
RMSD bond angle	0.956
Data scaling software	HKL-3000
Phasing software	PHASES
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.780
High resolution limit [Å]	2.730	2.730
Rpim	0.049	0.585
Number of reflections	36917	1669
<I/σ(I)>	18.8	1
Completeness [%]	98.8	91.9
Redundancy	3.6	2.7
CC(1/2)		0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.8M Succinic acid