5YU6
CRYSTAL STRUCTURE OF EXPORTIN-5:RANGTP COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-08 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.560, 302.678, 88.264 |
| Unit cell angles | 90.00, 109.24, 90.00 |
Refinement procedure
| Resolution | 48.977 - 2.997 |
| R-factor | 0.2209 |
| Rwork | 0.218 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a6p |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.506 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.330 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 | 0.386 |
| Number of reflections | 55602 | 5227 |
| <I/σ(I)> | 11.8 | 2.4 |
| Completeness [%] | 82.3 | 77.7 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG3350, DTT, MgCl2, spermine tetrahydrochloride |
