5YSO
Crystal structure of Estrogen Related Receptor-3 (ERR-gamma) ligand binding domain with DN200434
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.886, 79.828, 106.714 |
Unit cell angles | 90.00, 107.81, 90.00 |
Refinement procedure
Resolution | 43.711 - 2.501 |
R-factor | 0.1992 |
Rwork | 0.197 |
R-free | 0.24340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s9q |
RMSD bond length | 0.008 |
RMSD bond angle | 0.938 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.591 |
High resolution limit [Å] | 2.501 | 2.501 |
Rpim | 0.043 | 0.141 |
Number of reflections | 28403 | |
<I/σ(I)> | 2.6 | 8.2 |
Completeness [%] | 96.5 | 76.67 |
Redundancy | 6.8 | 6.9 |
CC(1/2) | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M Ammonium fluoride, 20%(w/v) PEG 3350, 10mM Barium chloride dihydrate |