5YOY
Crystal structure of the human tumor necrosis factor in complex with golimumab Fv
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 133.640, 85.300, 135.960 |
Unit cell angles | 90.00, 101.61, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.250 | 46.250 | 2.800 |
High resolution limit [Å] | 2.730 | 12.200 | 2.730 |
Rmerge | 0.107 | 0.046 | 0.988 |
Rmeas | 0.127 | 0.055 | 1.163 |
Total number of observations | 267101 | ||
Number of reflections | 77915 | 908 | 5439 |
<I/σ(I)> | 9.47 | 21.46 | 1.48 |
Completeness [%] | 97.1 | 94.7 | 92.8 |
Redundancy | 3.428 | 3.305 | 3.52 |
CC(1/2) | 0.993 | 0.997 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M sodium acetate trihydrate pH 4.6, 1.4M sodium chloride |