5YL4

CRYSTAL STRUCTURE OF T2R-TTL-8WR COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	105.461, 157.730, 182.147
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.225 - 2.640
R-factor	0.21954
Rwork	0.218
R-free	0.25591
RMSD bond length	0.008
RMSD bond angle	1.307
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.594
Number of reflections	93390
<I/σ(I)>	11.6
Completeness [%]	99.8
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	6% POLYETHYLENE GLYCOL 4000, 8% GLYCEROL, 0.1M MES, 30MM CACL2, 30MM MGCL2, PH 6.7, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K