5YHW
Crystal structure of Pig SAMHD1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-12 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 |
Unit cell lengths | 86.540, 95.909, 130.749 |
Unit cell angles | 78.66, 88.12, 82.68 |
Refinement procedure
Resolution | 29.944 - 2.700 |
R-factor | 0.2635 |
Rwork | 0.263 |
R-free | 0.29040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bzc |
RMSD bond length | 0.004 |
RMSD bond angle | 0.659 |
Data reduction software | PHENIX ((1.10.1_2155)) |
Data scaling software | PHENIX ((1.10.1_2155)) |
Phasing software | PHENIX ((1.10.1_2155)) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.787 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 108746 | |
<I/σ(I)> | 11.8 | |
Completeness [%] | 96.9 | 96.1 |
Redundancy | 3.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Sodium acetate trihydrate pH 7.0, 20% w/v Polyethylene glycol 3,350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |