5YEG
Crystal structure of CTCF ZFs4-8-Hs5-1a complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.167, 56.502, 67.486 |
| Unit cell angles | 78.41, 79.31, 78.60 |
Refinement procedure
| Resolution | 39.838 - 2.000 |
| R-factor | 0.1872 |
| Rwork | 0.186 |
| R-free | 0.20420 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.004 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.840 | 39.840 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.370 |
| Rpim | 0.035 | 0.210 |
| Number of reflections | 42594 | |
| <I/σ(I)> | 20.9 | |
| Completeness [%] | 97.1 | 95.6 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289.15 | 0.1M Bis-tris pH 5.6-6.0, 0.2M Sodium chloride, 17-24% PEG3350 |
