5YE4
Crystal structure of the complex of di-acetylated histone H4 and 1A9D7 Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.346, 164.093, 73.700 |
Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
Resolution | 37.761 - 1.799 |
R-factor | 0.1944 |
Rwork | 0.194 |
R-free | 0.22420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fj1 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.710 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 287775 | |
<I/σ(I)> | 28.3 | |
Completeness [%] | 99.0 | 96.8 |
Redundancy | 3.5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM imidazole (pH 6.5), 150 mM zinc acetate, and 17.5% (w/v) PEG3000 |