5YDR
Structure of DNMT1 RFTS domain in complex with ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 65 |
Unit cell lengths | 131.760, 131.760, 61.025 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.769 - 2.003 |
R-factor | 0.2001 |
Rwork | 0.199 |
R-free | 0.23250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3av4 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.415 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2067) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.500 | 2.003 |
Rmerge | 0.105 | 0.478 |
Number of reflections | 79146 | |
<I/σ(I)> | 5.7 | |
Completeness [%] | 99.3 | |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | 291 | 20mM Tris-HCl, 200mM sodium acetate, 25% PEG 4000 |