5YBF
Crystal structure of the GluA2o LBD in complex with glutamate and HBT1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-22 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 113.596, 162.074, 47.103 |
Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.500 |
R-factor | 0.18536 |
Rwork | 0.184 |
R-free | 0.20796 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ftj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.389 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 269829 | 17864 |
<I/σ(I)> | 18.2 | 2.2 |
Completeness [%] | 99.5 | 98.7 |
Redundancy | 3.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.9 | 277 | 15% PEG 3350, 0.1 M sodium acetate, 0.1 M zinc acetate |