5Y9E

Crystal structure of HPV58 pentamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2014-09-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	187.213, 101.804, 136.195
Unit cell angles	90.00, 95.68, 90.00

Refinement procedure

Resolution	29.781 - 2.042
R-factor	0.1707
Rwork	0.168
R-free	0.19830
RMSD bond length	0.002
RMSD bond angle	0.525
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.040
Number of reflections	159674
<I/σ(I)>	19.6
Completeness [%]	99.2
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M magnesium formate, 14.5% (w/v) PEG 3350