5Y7X

Human Peroxisome proliferator-activated receptor (PPAR) delta in complexed with a potent and selective agonist

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	39.766, 93.896, 96.871
Unit cell angles	90.00, 97.40, 90.00

Refinement procedure

Resolution	29.655 - 1.699
R-factor	0.2061
Rwork	0.205
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u3r
RMSD bond length	0.006
RMSD bond angle	0.790
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.800	1.760
High resolution limit [Å]	1.699	1.700
Rmerge	0.066	0.963
Number of reflections	76750
<I/σ(I)>	32.5
Completeness [%]	98.9
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	10% PEG 8000, 40mM Bis-Tris propane pH 8.5, 2.5% 1,2-propanediol, 200mM KCl, 10mM DTT