5Y6U
Crystal structure of wild-type YabJ protein from Bacillus subtilis (natto).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-06-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 |
| Unit cell lengths | 53.767, 53.767, 203.941 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.560 - 1.500 |
| R-factor | 0.1616 |
| Rwork | 0.161 |
| R-free | 0.18024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qd9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.169 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.064 | 0.554 |
| Number of reflections | 53138 | 2636 |
| <I/σ(I)> | 57.6 | 6.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.2 | 21.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 22.5% PEG 3350, 0.45 M ammonium acetate, 0.1 M sodium acetate buffer pH 4.2 |
