5Y6F
Crystal structure of YcgR in complex with c-di-GMP from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | H 3 |
Unit cell lengths | 93.980, 93.980, 109.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.990 - 2.300 |
R-factor | 0.1855 |
Rwork | 0.183 |
R-free | 0.23340 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.380 |
Data reduction software | iMOSFLM (v.7.0.4) |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.990 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.054 | 0.493 |
Rpim | 0.026 | 0.232 |
Number of reflections | 15972 | 2357 |
<I/σ(I)> | 15 | 3.1 |
Completeness [%] | 99.5 | 100 |
Redundancy | 5.4 | 5.5 |
CC(1/2) | 0.998 | 0.818 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.2M potassium thiocyanate, 0.1M Bis-Tris pH 6.0, 20% (w/v) PEG 3350 |