5Y4H
Human SIRT3 in complex with halistanol sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 127.840, 127.840, 127.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.21793 |
| Rwork | 0.215 |
| R-free | 0.26914 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3glu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.515 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 11529 |
| <I/σ(I)> | 88.8 |
| Completeness [%] | 99.9 |
| Redundancy | 16.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Potassium phosphate |
