5XUZ

Crystal structure of Lachnospiraceae bacterium ND2006 Cpf1 in complex with crRNA and target DNA (CCCA PAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	101.946, 103.540, 342.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.851 - 2.400
R-factor	0.1801
Rwork	0.177
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xus
RMSD bond length	0.007
RMSD bond angle	0.883
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.851	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	138250
<I/σ(I)>	9.2
Completeness [%]	97.3
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	PEG3350, MIB buffer, pH 5.0