5XTZ

Crystal structure of GAS41 YEATS bound to H3K27ac peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	75.864, 80.663, 107.028
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.103 - 2.105
R-factor	0.1955
Rwork	0.194
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tmp
RMSD bond length	0.008
RMSD bond angle	1.109
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	38636
<I/σ(I)>	16.8
Completeness [%]	99.6
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	0.1 M sodium acetate trihydrate, pH 4.6, 4.0 M ammonium acetate