5XPU
Crystal structure of MAD2L2/REV7 in complex with a CAMP fragment in a monoclinic crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.025, 70.562, 45.131 |
| Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
| Resolution | 19.090 - 2.304 |
| R-factor | 0.2202 |
| Rwork | 0.220 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3abe |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.269 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.090 | 2.430 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 9883 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.3 M potassium thiocyanate, 20% PEG 3350 |
