5XPG

Crystal structure of T. thermophilus Argonaute protein complexed with a bulge 6'U7' on the target strand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97926
Spacegroup name	P 41 3 2
Unit cell lengths	202.351, 202.351, 202.351
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.080 - 2.800
R-factor	0.205
Rwork	0.203
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dlh
RMSD bond length	0.007
RMSD bond angle	1.201
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (PHENIX.REFINE)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.086	0.389
Number of reflections	35223
<I/σ(I)>	21.6	2.2
Completeness [%]	99.5	98.3
Redundancy	8.7	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	308	1.0 M (NH4)2SO4, 0.1 M KCL, 10 MM MGCL2, 50 MM BIS-TRIS PH 6.5 , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 308K