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5XN0

HIV-1 reverse transcriptase Q151M:DNA binary complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPHOTON FACTORY BEAMLINE BL-17A
Synchrotron sitePhoton Factory
BeamlineBL-17A
Temperature [K]100
Detector technologyPIXEL
Collection date2016-12-15
DetectorDECTRIS PILATUS3 6M
Wavelength(s)0.98000
Spacegroup nameH 3
Unit cell lengths284.091, 284.091, 95.605
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution48.605 - 2.596
R-factor0.1868
Rwork0.185
R-free0.22630
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5d3g
RMSD bond length0.009
RMSD bond angle1.142
Phasing softwareMOLREP
Refinement softwarePHENIX ((1.11.1_2575))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.750
High resolution limit [Å]2.5962.600
Rpim0.084
Number of reflections88842
<I/σ(I)>16.9
Completeness [%]100.0
Redundancy5.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP629320 mM bis-Tris-HCl pH 6.0, 60 mM di-ammonium hydrogen citrate, 20 mM MgCl2, 1.5-3% PEG 6000, 2-4% sucrose, 4-8% glycerol

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