5XJL
Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1/D2/F/E/G from Human
Replaces: 3S6NExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99993 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.830, 84.600, 104.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.190 - 2.500 |
R-factor | 0.2238 |
Rwork | 0.220 |
R-free | 0.29570 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 59.190 |
High resolution limit [Å] | 2.500 |
Number of reflections | 19831 |
<I/σ(I)> | 13.7 |
Completeness [%] | 99.3 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 1%PEG8000, 100mM Tris-HCl |