5XJC

Cryo-EM structure of the human spliceosome just prior to exon ligation at 3.6 angstrom

This is a non-PDB format compatible entry.

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Quantum LS
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.6
Software	RELION
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION