5XIU

Crystal structure of RNF168 UDM2 in complex with Lys63-linked diubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-06-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 2
Unit cell lengths	59.442, 78.548, 31.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.1713
Rwork	0.169
R-free	0.20690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fid
RMSD bond length	0.006
RMSD bond angle	0.741
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	14042
<I/σ(I)>	20.7	2.7
Completeness [%]	99.3	99.2
Redundancy	7.7	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	25% PEG3350, 0.1 M Tris-HCl (pH8.5), 0.2 M Ammonium Acetate