5XHC

Crystal structure of T2R-TTL-PO10 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	105.257, 157.885, 182.427
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	119.380 - 2.750
R-factor	0.22993
Rwork	0.227
R-free	0.27682
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fnv
RMSD bond length	0.007
RMSD bond angle	1.237
Data reduction software	SCALEPACK
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	119.380
High resolution limit [Å]	2.750
Number of reflections	78443
<I/σ(I)>	0
Completeness [%]	99.8
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7